Like the title says, which scenes from the Italian westerns really tug at your heart strings?
There are several for me:
A Pistol For Ringo- That scene at the very end when it shows the older gentleman (forgot his name) walking over to Nieves Navarro's character's body was very effective to me. I think it was just seeing that sad look on his face and the music really enhanced that moment.
The Good The Bad and The Ugly- Of course the scene where Blondie lets that dying soldier have a few puffs of his cigar before he dies.
The Great Silence- There were a lot in this movie, but I think for me the most emotional is when the mother of the young refugee who got shot down by Charlie begs Silence to go and kill him. The music again really enhanced this scene for me.
Companeros- Not too incredibly sad, but still when Professor Xantos gets shot down by John, the fact that he stood up to him with an unloaded gun just seemed so brave and made his death that much more powerful.
Once Upon a Time in the West- There were several in this film, so I'll leave that open for discussion.
Duck You Sucker- Same as above.
There are so many that I can think of, but I'll let wait until some more people give their input.